关键词: 山羊, 卵母细胞, 体外培养成熟

Abstract: In this experiment fresh ovary from slaughtered goats is used as the material.By making use of the method of taking out oocytes with incise to wash the egg method combines together, recovering cumulus oocytes complexes(COC) can be educated, recovery rate is 1.43/ovary.This kind of COC is placed in M199+the FBS(10%) educated liquid+FSH(10 IU/mL)+LH(20 μg/mL) educated tiny inside, in the CO₂ educated box of 5% CO₂, 95% air, saturated degree of humidity, the different temperature descends to educate 24~26 h.In 38.0℃, 38.5℃, 39.0℃ different temperatures, the maturation rate distinguishes to 82.1%, 81.6%, 83.3%.The result expresses as long as the educated temperature is within the scope of the variety of the normal body temperature in goat, then the maturation rate in vitro has no obvious difference.

Key words: goat, oocytes, IVM