关键词: 转基因抗虫棉, 棉铃虫, 抗性鉴定, 卡那霉素

Abstract: The identification method of transgenic cotton by using kanamycin as an indirect marker is presented.Dropping with 8000 mg/kg kanamycin solution on cotyledon in seedling stage, or with 6000 mg/kg kanamycin solution on the second expand leaves in bud stage, 7~9 days later, all of the leaves of non transgenic cotton plants show apparent yellow patches whereas the leaves of the transgenic cotton remain green without any symptoms.By using this method, the transgenic cotton plant can be identified effectively and simply, and the results are in agreement with results obtained by both the cage inoculating test and the insect resistance bioassay test.

Key words: transgenic cotton, cotton bollworm, resistance identification, kanamycin