关键词: Glu-1位点, Gli-B1l位点, 十二烷基磺酸钠聚丙烯酰胺凝胶电泳, 酸性聚丙烯酰胺凝胶电泳, 高分子量谷蛋白亚基, 醇溶蛋白

Abstract: HMW-GS composition at Glu-1 loci was analysed in 26 accessions of two advanced resistant wheat lines derived from wheat rye hybrids by using sodium dodeyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE), Gliadin Gli-B1l loci identified by acid polyacrylamide gel electrophoresis(A-PAGE). From the glutenin patterns, 2, 3, 2 alleles were identified at Glu-A1, Glu-B1 and Glu-D1 loci, respectively in these materials. At each locus, the highest frequency alleles were Glu-A1a(96.15%), Glu-B1b(92.30%) and Glu-D1d(84.62%). The Nei's genetic variation indexes(H) indicate that the highest genetic diversity is found at Glu-D1 loci, the higher is found at Glu-B1 loci, while the lowest is Glu-A1 loci; the H of 98-1104 line at Glu-A1, Glu-B1 and Glu-D1 loci are 0, 0.180, 0.180, correspondingly; for 98-917 line, the H at three loci are 0.117, 0.117, 0.305 correspondingly. Further HMW-GS combination analysis of advanced resistant wheat lines shows there are five types:(1, 7+8, 5+10), (1, 7+9, 2+12), (1, 7+8, 2+12), (N, 7+8, 5+10), (1, 14+15, 5+10); therein the highest frequency composition is the model of Parent A42912(1, 7+8, 5+10). The gliadin patterns indicate that four variable materials at Glu-1 loci:98-1104-9(1, 7+9, 2+12), 98-917-15(1, 7+8, 2+12), 98-917-27(N, 7+8, 5+10), 98-917, 29(1, 14+15, 5+10) are 1RS/1BL translocation lines. Ultimately the mechanism of allele variation of Glu-1 loci1induced by 1RS/1BL translocation is discussed.

Key words: GLU-1 LOCI, GLI-B1L LOCI, SDS-PAGE, A-PAGE, HMW-GS, GLIADIN