四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (03): 277-280.doi: 10.16036/j.issn.1000-2650.2001.03.024

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毕赤巴斯德酵母表达外源蛋白研究进展

马孟根, 王红宁   

  1. 四川农业大学 动物科技学院, 四川 雅安 625014
  • 收稿日期:2001-02-22 出版日期:2001-09-30 发布日期:2017-03-06
  • 基金资助:
    国家"九五"科技攻关项目(99-009-02-02)

Advance in the Expression of Foreign Protein in Pichia Pastoris

MA Meng-gen, WANG Hong-ning   

  1. College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2001-02-22 Online:2001-09-30 Published:2017-03-06

摘要: 毕赤巴斯德酵母表达系统具有真核生物表达的特点,现已广泛用于外源蛋白的表达。本文综述了其自身的优点,表达载体的特点,外源基因拷贝数的多少与表达产量的关系,分泌型蛋白的表达,表达外源蛋白所需的外部条件以及翻译后的加工和修饰过程。

关键词: 毕赤巴斯德酵母, 外源蛋白, 高效表达

Abstract: P. pastoris possesses the advantage of eukaryotic cell, and has been used to express foreign proteins widely. The advantage of P. pastoris, the characteristic of expression plasmid, the relationship between copies of foreign gene and foreign protein yield, expression of secreted protein, condition of fermentation and processing, modifying protein procedures are discussed in this paper.

Key words: P. PASTORIS, FOREIGN PROTEIN, HIGH-LEVEL EXPRESSION

中图分类号: 

  • Q786