四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (03): 266-268.doi: 10.16036/j.issn.1000-2650.2001.03.021

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小鼠腔前卵泡的分离采集和体外成熟研究

郑鸿培1, 曾长军1, 张安居2, 侯容2, 张志和2, 张美佳2, 兰景超2, 王基山2, 何萍2, 张瑛2   

  1. 1. 四川农业大学 动物科技学院, 四川 雅安 625000;
    2. 成都大熊猫繁育研究基地, 四川 成都 610081
  • 收稿日期:2001-06-06 出版日期:2001-09-30 发布日期:2017-03-06
  • 基金资助:
    成都大熊猫繁育基金会资助项目

Isolation and in Vitro Maturation of Mouse Preantral Follicles

ZHENG Hong-pei1, ZENG Chang-jun1, ZHANG An-ju2, HOU Rong2, ZHANG Zhi-he2, ZHANG Mei-jia2, LAN Jing-chao2, WANG Ji-shan2, HE Ping2, ZHANG Yin2   

  1. 1. College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625000, Sichuan, China;
    2. Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, Sichuan, China
  • Received:2001-06-06 Online:2001-09-30 Published:2017-03-06

摘要: 采用机械法和酶法结合机械法分离采集小鼠卵巢,每只小鼠可获腔前卵泡53.25和57.29枚(P<0.05)。成年小鼠腔前卵泡在体外培养9d,换用成熟培养液继续培养24~28h后,3种不同培养系统中卵泡的成熟分裂率分别为5.47%、3.85%和9.31%。表明EMEM+5% FCS+0.23mmol/mL丙酮酸钠+20μg/mLFSH+0.05mmol/mL次黄嘌呤+200IU/mL青-链霉素培养系统可以使小鼠腔前卵泡卵母细胞在体外发育达到成熟。

关键词: 小鼠, 腔前卵泡, 体外成熟

Abstract: In present experiment, the preantr8l follicles are isolated from mouse ovaries by mechanical and enzyme digestion combined with mechanical treatments. The average number of preantral follicles, collected by two treatments, are 53.25 and 57.29 preamral follicles per pair of ovaries(P<0.05). Maturation rate of three culture systems are 5.47%, 3.85% and 9.31% for 9 days culture. There are significant differences among the three culture systems(P<0.05). It appears that EMEM+5% FCS+0.23mmol/mL sodium pyruate+20μg/mL FSH+0.05mmol/mL Hypoxahine+200IU/mL penicillin streptomycin is suitable for in vitro maturation mouse preanral follicles.

Key words: MOUSE, ISOLATION IN VITRO MATUATION, PREANTRAL FOLLICLE

中图分类号: 

  • Q813.7