四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (03): 197-199,217.doi: 10.16036/j.issn.1000-2650.2001.03.002

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粗山羊草高分子量麦谷蛋白新型亚基的筛选和鉴定

颜泽洪1,2, 郑有良1, 万永芳2, 刘坤凡2, 王道文2   

  1. 1. 四川农业大学 小麦研究所, 四川 都江堰 611830;
    2. 中国科学院 遗传研究所, 北京 100101
  • 收稿日期:2001-07-11 出版日期:2001-09-30 发布日期:2017-03-06
  • 基金资助:
    四川省科技厅科研项目;四川省教育厅科研项目

Identification of Novel High-molecular-weight Glutenin Subunits from Aegilops Tauschii

YAN Ze hong1,2, ZHENG You liang1, WAN Yong fang2, LIU Kun fan2, WANG Dao wen2   

  1. 1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangy an 61830, Sichuan, China;
    2. Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101, China
  • Received:2001-07-11 Online:2001-09-30 Published:2017-03-06

摘要: 利用SDS-PAGE和Western Blotting分析与鉴定技术,从51份粗山羊草中共发现了2种新型亚基Tx >2和Ty <12,被命名为Dx2.1t和Dy13t;7种亚基组合类型2+12、5+10、2+10、5+12、2.1t+10、2.1t+12和2+13t,这7种亚基组合的频率分别是27.5%、25.5%、31.4%、3.9%、2.0%、2.0%和7.8%。已将新型亚基Dx2.1t和Dy13t的编码基因进行了分子克隆。

关键词: 粗山羊草, 小麦, 高分子量麦谷蛋白亚基

Abstract: The high-molecular-weight glutenin subunits compositions of 51 accessions of Aegilops tauschii were investigated by using SDS-PAGE analysis and Western blotting technical. Two novel types of high-molecular-weight glutenin subunits, Tx>2(with mobility faster than the Dx2 and Dx5 of common wheat) and Ty<12(with mobility slower than the Dy10 and Dy12 of common wheat) were observed, which were designated as Dx2.1t and Dy13t, respectively. The frequencies of 7 subunit combinations(2+12, 5+10, 2+10, 5+12, 2.1t+10, 2.1t+12 and 2+13t) were 27.5%, 25.5%, 31.4%, 3.9%, 2.0%, 2.0%和7.8%, respectively. We have cloned the novel high-molecular-glutenin subunits Dx2.1t and Dy13t.

Key words: AEGILOPS TAUSCHII, TRITICUM AESTIVUM, HIGH MOLECULAR WEIGHT GLUTNIN SUBUNIT

中图分类号: 

  • S512.1