四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (02): 141-143.doi: 10.16036/j.issn.1000-2650.2001.02.012

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西洋杜鹃组织培养技术体系研究(Ⅱ)——培养物的增殖和生根

钟宇, 张健, 罗承德, 陈其兵   

  1. 四川农业大学 生态林业工程省级重点实验室, 四川 雅安 625014
  • 收稿日期:2001-02-15 出版日期:2001-06-30 发布日期:2017-03-06

A Study on the Technique System of Tissue Culture in Rhododendron Hybridn(Ⅱ)——Study on the Propagation and Rooting of Cultures

ZHONG Yu, ZHANG Jian, LUO CHENG-de, CHEN Qi-bing   

  1. Laboratory of Ecological Forestry Engineering the Provincial Key Laboratory, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2001-02-15 Online:2001-06-30 Published:2017-03-06

摘要: 研究西洋杜鹃茎尖再生植株无菌培养物建立、初代培养、继代培养和生根培养4个技术环节,最终建立西洋杜鹃组培快速繁殖技术体系。试验结果按最佳处理计算,一个外植体年理论增殖系数可达到2.6×105。植物组培快繁技术是解决西洋杜鹃种苗缺乏的有效途径。

关键词: 西洋杜鹃, 组织培养, 快速繁殖

Abstract: A rapid propagation method of R. hybridn via tissue culture includes establishment of sterile cultures, primary culture, subculture and rooting culture. Theoretically, the rate of propagation of a shoot tip can reach 2 6×105 for one year. The rapid propagation via tissue culture is a effective method solving the short of seeding of R. hybridn.

Key words: R. HYBRIDN, TISSUE CULTURE, RAPID PROPAGATION

中图分类号: 

  • S731.01