四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (01): 37-39.doi: 10.16036/j.issn.1000-2650.2001.01.010

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西洋杜鹃组织培养技术体系研究(Ⅰ)——基本培养基和外植体的选择

钟宇, 张健, 罗承德, 陈其兵   

  1. 四川农业大学 林学园艺学院, 四川 雅安 625014
  • 收稿日期:2001-02-15 出版日期:2001-03-15 发布日期:2017-03-06

A Study on the Technique System of Tissue Culture in Rhododendron Hybrind(Ⅰ)——Selection of Medium and Explant

ZHONG Yu, ZHANG Jian, LUO Cheng-de, CHEN Qi-bing   

  1. College of Forestry and Horticulture, Sichuan Agriculture University, Yaan 625014, Sichuan, China
  • Received:2001-02-15 Online:2001-03-15 Published:2017-03-06

摘要: 实验以不同季节的茎尖(含茎段)为材料,采用不同基本培养基,研究确定西洋杜鹃组织培养适合的基本培养基类型,植株再生的可能途径,以及适宜的培养条件。结果表明:①低盐分浓度及高比值NH4+/NO3-的基本培养基Read培养基适合西洋杜鹃;②最适外植体为摘花芽后萌发的顶芽茎尖;③最佳取材时间为3~5月;④培养条件:温度25±2℃,光照16h/黑暗8h,光照强度1500~2500lx。

关键词: 西洋杜鹃, 组织培养, 快速繁殖

Abstract: In view of studying the suitable type of medium, probable way of explant regeneration and suitable condition of culture for tissue culture in R. hybridn, a experimentation was established in which shoot tip from different seasons and a series of different medium were employed. The results are as follows:①Read medium with low mineral salt concentration and high ration of NH4+/NO3- is suitable for R. hybridn microculture; ②The optimal explants are shoot tips of end buds excised from R. hybridn in 3~5 months; ③All the cultures are kept under the temperature of 25±2℃, illuminated for 16 hours per day, and light intensity was 1500~2500lx.

Key words: R. HYBRIDN, TISSUE CULTURE, RAPID PROPAGATION

中图分类号: 

  • S723.132