四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (01): 31-33.doi: 10.16036/j.issn.1000-2650.2001.01.008

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球孢白僵菌液体制剂研究

朱天辉1, 杨世璋2, 李远翔2   

  1. 1. 四川农业大学 林学园艺学院, 四川 雅安 625014;
    2. 重庆市森林病虫防治检疫总站, 重庆 永川 402160
  • 收稿日期:2000-10-17 出版日期:2001-03-15 发布日期:2017-03-06
  • 基金资助:
    四川省林业厅《白僵菌生产新工艺的研究》课题的部分内容

The Study on A Liquid Preparation of Beauveria Bassiana

ZHU Tian-hui1, YANG Shi-zhang2, LI Yuang-xiang2   

  1. 1. College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    2. Chongqing general Station for Controlling Forest Disease and Pest, Yongchuan 402160, Chongqing, China
  • Received:2000-10-17 Online:2001-03-15 Published:2017-03-06

摘要: 通过液体发酵试验,研究了不同营养基质、不同发酵条件对球孢白僵菌液生分生孢子、芽孢子、干物质积累的影响,结果显示:在5种培养基中,除"糖蜜5%、玉米浸提液1%、K2HPO4 0.1%"(代号D)外,其它均不产生芽孢子,以D配方生物量最高,孢子较大,为本试验选出的液体制剂的理想载体,最佳发酵条件为温度25~30℃,pH6.0~7.0,通气量以瓶装培养液量为指标不超过1/6瓶装量,发酵时间以96小时为佳。白僵菌液体制剂的理想载体与最佳发酵条件的组合可使白僵菌液生分生孢子达2.5×1010左右,超过了目前四川固体发酵的生产水平。

关键词: 白僵菌, 液体发酵, 分生孢子, 生物防治

Abstract: Effects of different media and fermentative conditions on the production of spores and cumulation of mycelial substence for Beauveria bassiana were studied by means of liquid fermentation. The results show that Beauveria bassiana does not produce blastospores on five media except for the medium(substitute code D) which contains 5% waste sugar, 1% corn abstract, and 0.1% K2HPO4, meanwhile, the D-medium, on which Beauveria bassiana grows well and produces bigger spores, is regarded as a ideal liquid carrier. The optimal fermentative conditions are as follows:temperature 25~30℃, pH6.0~7.0, Vibrating time 96hr, and not excess 1/6 flak volume as ventilation index.

Key words: BEAUVERIA BASSIANA, LIQUID FERMENTATION, CONIDIA, BIOLOGICAL CONTROL

中图分类号: 

  • S763.306.4