关键词: Dot-PPA-ELISA, 联合检测, 抗体, 应用

Abstract: A Dot-PPA-ELISA was developed for the co-detection of swine Brucella, PRV and Chlamydia antibodies for the first time and the capability of it's relatively large scale appliance was determined. In this study was careful study carried through in technecs flow of diaphragm making and the key matiarial. The best reacting condition and other important reagent were determined. The co-detection diaphragm does not react with the antibodies of swine fever, swine foot-mouth disease and swine bow-shaped body disease. The minium amount of Prv, Brucella and Chlamydia serum IgG giving a positive reaction is 5.326×10^-9 g, 7.221×10^-9 g and 1.21×10^-9 g. 12 serum samples are detected for 3 times, making T test, the result is P>0.05. The diaphragm stored at room tenperature for 2 monmths, at 4℃ at least for 8 months, at 37℃ for 1 months, the sensitivity and specificity did not change. The positive standards of the diaphragm of Prv, Brucella and Chlamydia antibodies were 1:128, 1:64 and 1:64. All the results show the co-detection diaphragm is specific, stable, rapid detection. It can be used in co-detecting the Prv, Brucella and Chlamydia antibodies.

Key words: Dot-PPA-ELISA, CO-DETECTION, ANTIBODY, APPLICATION