四川农业大学学报 ›› 1999, Vol. 17 ›› Issue (03): 254-260.doi: 10.16036/j.issn.1000-2650.1999.03.004

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四川小麦主栽品种醇溶蛋白遗传差异分析

陈玉清, 郑有良, 魏育明   

  1. 四川农业大学 小麦研究所, 四川 都江堰 611830
  • 收稿日期:1999-08-25 出版日期:1999-09-30 发布日期:2017-04-22
  • 基金资助:
    四川省科委及四川省教委科研项目

The Study on Gliadin Variation of Sichuan Major Wheat Cultivars

CHEN Yuqing, ZHENG Youliang, WEI Yuming   

  1. Triticeae Research Institute of Sichuan Agricultural University, Dujiangyan 611830, China
  • Received:1999-08-25 Online:1999-09-30 Published:2017-04-22

摘要: 采用酸性聚丙烯酰胺凝胶电泳(APAGE)对四川省近50年来年推广面积6.66万hm2以上的40个小麦主栽品种进行醇溶蛋白位点特异性检测,分析了不同基因型间醇溶蛋白的遗传差异。结果表明:40个品种具有38种醇溶蛋白带型,其中9个品种具有1BL/1RS易位标记位点Gli-Bll;醇溶蛋白电泳分离出的46条带中,40条具有多态性,占84.8%。供试品种间遗传距离(GD)在0~0.67之间,平均值为0.33,遗传变异较大,特别是早期品种与近20年育成品种间,以及1BL/1RS易位系品种与非1BL/1RS易位系品种间均具有较大的遗传距离。聚类分析表明:供试品种在遗传距离0.35水平上明显聚为4类,具有相同血缘的品种大多数聚在了同1类。分析认为,四川小麦品种醇溶蛋白变异丰富,存在广泛的遗传多样性

关键词: 小麦, 品种, 醇溶蛋白, 遗传距离, 聚类分析

Abstract: In order to investigate the special variation of gliadin alleles and analyse the genetic difference between different genotypes, 40 cultivars which had once popularized above 1,000,000mu per year in Sichuan during the recent 50 years were used in this study with acid polyacrylamide gel electrophoresis (APAGE). The results were as follows:There were 38 gliadin genotypes in the 40 cultivars, with 9 cultivars bearing the allele Gli Bll, a marker of 1BL/1RS translocation. 46 gliadin bands were seperated by electrophoresis and 40 of them showed polymorphism (amount to 84.8%). The genetic distance (GD) based on gliadin ranged from 0.to 0.67, with an average of 0.33. There were relatively higher GD between the early and recent 20 years cultivars of 1BL/1RS and non 1BL/1RS translocation lines. Cluster analysis shows that the studied cultivars could be classified into 4 clusters at the level of GD 0.35, and the majority of variaties with same consanguinity are clustered together. Based on these results, it is concluded that Sichuan wheat cultivars have abundant variation and high genetic diversity.

Key words: WHEAT (T. aestivum L., CULTIVARS, GLIADIN, GENETIC DISTANCE, CLUSTER ANALYSIS

中图分类号: 

  • S512.1(271)