四川农业大学学报 ›› 1999, Vol. 17 ›› Issue (01): 55-57.doi: 10.16036/j.issn.1000-2650.1999.01.011

• 研究论文 • 上一篇    下一篇

用PCR-RFLP技术鉴别猪的氟烷基因型

郭雁君1, 罗安治1, 陈继华2, 肖艳玲2, 季永诚2   

  1. 1. 四川农业大学动物科技学院, 雅安市 625014;
    2. 乐山市种畜场, 四川乐山 614004
  • 收稿日期:1998-11-03 出版日期:1999-03-31 发布日期:2017-04-22
  • 基金资助:
    四川省"九五"攻关项目

IDENTIFICATION ON PORCINE HALOTHANE GENOTYPES BY POLYMEROSE CHAIN REACTION

Guo Yanjun1, Luo Anzhi1, et al2   

  1. College of Animal Science and Technology, Sichuan Agricultureal University, Yaan, Sichuan, China 625014
  • Received:1998-11-03 Online:1999-03-31 Published:2017-04-22

摘要: 用PCR-RFLP技术对乐山市种畜场长白猪选育基础群的氟烷基因型进行了检测,查清了Haln基因在该群体中的分布,Haln基因的频率为18.056%。同时对影响PCR的几个主要因素进行了初步研究。

关键词: 猪, PCR-RFLP技术, 氟烷基因

Abstract: Porcine Stress Syndrome(PSS) is controlled by Halothanegene located on porcine No.6 chromosome. Identification of the porcineHalothane genotypes was established by PCR-RFLP. The Halothane genefragment containing the mutation point was amplified and the PCR product was incubated with restiction endonuclease HhaI, then genotypes were detected. And several factors that effected PCR are analysised.

Key words: PORCINE STRESS SYNDROME, HALOTHANE GENOTYPE, POLYMERASECHAIN REACTION (PCR)

中图分类号: 

  • S828.2