关键词: 腔前卵泡, 卵母细胞, 超微结构, 山羊

Abstract: Oocytes from preantral follicles in goat cultured for 0, 7 and 16 days were fixed in 2.5% glutaraldehyde soluton for the preparation of electron microscopic samples. Electron microscopic research shows that the ultrastructure of oocytes cultured for 7 days in vitro are basically normal, while those cultured for 16 days are variable in the sturcture. The characterization of CGs arrangement under plasma membrane are not observed in most oocytes cultured for 16 days. The mitochondria of some oocytes are still oblongus with lamellar cristae and the organelle migrated abnormally. In other oocytes, it was identified that part of nuclear membrane began to disappear, during which GVBD occurred. The degenerating polar body could be easily distinguished in a parthenogenetic egg. From the findings of electron microscopic research, we suppose that the metabolism in oocytes can be partly abnormal by the end of culture, which prevents the synthesis of some components (such as CGs) or migration of some organelle. Therefore, the late stage of culture is the key period influencing the maturation of oocyte.

Key words: PREANTRAL FOLLICLE, OOCYTE, ULTRASTUCTURE, GOAT