四川农业大学学报 ›› 1998, Vol. 16 ›› Issue (04): 439-443.doi: 10.16036/j.issn.1000-2650.1998.04.011

• 研究快报 • 上一篇    下一篇

月季组培快繁技术研究

莫磊兴, 牟海飞, 邹瑜, 李小泉, 林贵美   

  1. 广西农科院植物组培苗有限公司, 南宁 530007
  • 收稿日期:1998-06-10 出版日期:1998-12-31 发布日期:2017-04-21

STUDY ON TECHNIQUE FOR MICROPROPAGATION OF ROSE

Mo Leixing, Mou Haifei, Zou Yu, Li Xiaoquan, Lin Guimei   

  1. Guangxing in vitro plant Co. Ltd, Guangxi Academy of Agricultural Sciences, Nanning, China 530007
  • Received:1998-06-10 Online:1998-12-31 Published:2017-04-21

摘要: 通过对5个切花月季品种组培快繁技术的研究,探讨了品种、植物生长调节剂、温度和光照强度等对外植体腋芽萌发、嫩茎增殖和生根的影响。茎段腋芽诱导适宜的条件是:MS+BA3.0mg/L+NAA0.15mg/L,温度26℃左右。在MS+BA3.0mg/L+IAA0.15mg/L培养基上,嫩茎增殖达4倍以上。3000lux左右的自然散射光对增殖阶段形成健壮嫩芽有利。小苗移植到沙子与椰糠混合的基质上,成活率达80%以上。

关键词: 月季组培快繁, 品种, 植物生长调节剂, 温度, 光照强度

Abstract: Studies on influences of varieties, plant growth regulators, temperature and intensity of illumination on bud germination, mutiplication and rooting were carried out through the micropropagation of 5 varieties of rose. The results show that for growth initiation, the optimum medium is MS+BA 3.0mg/L+NNA 0.15mg/L, and temperature is about 26℃. Shoots cultured on the medium of MS+BA 3.0mg/L+IAA 0.15mg/L could be multiplicated by 4 fold and above. 3000lux of random natural light is useful for robust bud formation. 80% of survival rate could be obtained when plantlets are transplanted to the matrix consisting of sand and coconut husk.

Key words: MICROPROPAGATION OF ROSE, VARIETIES, PLANT GROWTH REGULATORS, TEMPERATURE, INTENSITY OF ILLUMINATION

中图分类号: 

  • Q945.52