四川农业大学学报 ›› 1997, Vol. 15 ›› Issue (03): 304-306.doi: 10.16036/j.issn.1000-2650.1997.03.003

• 研究论文 • 上一篇    下一篇

麦角固醇高产酵母菌的快速筛选方法

杨婉身, 梁如玉   

  1. 四川农业大学基础部、农学院, 雅安市 625014
  • 收稿日期:1997-01-21 出版日期:1997-09-30 发布日期:2017-03-07

A RAPID METHOD FOR THE SELECTION OF ERGOSTEROL-PRODUCTION YEAST

Yang Wanshen, Liang Ruyu   

  1. Department of Basic, Sichuan Agricultural University/Yaan, Sichuan, China 625014
  • Received:1997-01-21 Online:1997-09-30 Published:2017-03-07

摘要: 介绍一种快速、简便、可靠的筛选麦角固醇高产酵母菌株的方法。将自溶后的酵母菌用超声波破碎,用乙醚萃取其所含麦角固醇,利用乙酸酐在酸性条件下与麦角固醇生成绿色化合物的原理,可直接根据绿色深浅来判断菌株的优劣。

关键词: 酵母菌, 麦角固醇, 筛选, 乙酸酐

Abstract: A rapid, simple and reliable method for the selection of ergosterol-producting yeast was reported in this paper. The yeast was broken with ultrasonic wave, the ergosterol in which was extracted with ether. According to the principle of the gree compounds production of acetic anhydride and ergosterol in acid solution the good or bad yeast trains could ditermine directly on the basis of the deep or light gree.

Key words: YEAST, ERGOSTEROL, SELECTION, ACETIS ANHYDRIDE

中图分类号: 

  • Q93-33