四川农业大学学报 ›› 1997, Vol. 15 ›› Issue (03): 297-299.doi: 10.16036/j.issn.1000-2650.1997.03.001

• 研究论文 •    下一篇

高效液相色谱测定多种植物内源激素方法研究

谢君   

  1. 四川农业大学分析测试中心, 雅安市 625014
  • 收稿日期:1997-01-24 出版日期:1997-09-30 发布日期:2017-03-07

DETERMINATION OF PLANT INTERNAL HORMONES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Xie Jun   

  1. Analysis & Testing Center, Sichuan Agricultural University/Yaan, Sichuan, China 625014
  • Received:1997-01-24 Online:1997-09-30 Published:2017-03-07

摘要: 研究高效液相色谱法测定赤霉素(GA3)、3-吲哚乙酸(IAA)、脱落酸(ABA)、玉米素(ZT)和激动素(KT)等五种植物激素的条件;采用Sphersorb C18柱,乙腈-甲醇-0.6%乙酸流动相,检测波长254nm;建立了一种从植物中提取五种激素的样品处理方法,实际分析了植物样。

关键词: 植物激素[45ND, 49LD], 高效液相色谱

Abstract: This essay studies the determination method of gibberellin, 3-indoleacetic acid, abscisic acid, zeatin and kinetin in plant by high performance liquid chromatography. Chromatographic conditions are Spherisorb C18 with acetonitril-methanol-0.6% acetic acid as mobil phase, Uv detection at 254nm. Under the selected optimum chromatographic conditions, this method is simple and conveninent with a high sensitivity.

Key words: PHYTOHORMONES, HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

中图分类号: 

  • O657.72