四川农业大学学报 ›› 1997, Vol. 15 ›› Issue (01): 95-98.doi: 10.16036/j.issn.1000-2650.1997.01.022

• 研究论文 • 上一篇    下一篇

四川省四大地方品种猪的随机扩增多态DNA分析

黄勇富1,2, 张亚平1, 曾凡同2, 陈永久1, 邱祥聘2, 肖永祚2   

  1. 1. 中国科学院昆明动物研究所细胞与分子进化开放研究实验室, 昆明市 650223;
    2. 四川农业大学动物科技学院, 雅安市 625014
  • 收稿日期:1996-08-24 出版日期:1997-03-31 发布日期:2017-03-07
  • 基金资助:
    国家自然科学基金资助项目

STUDY ON RANDOM AMPLIFIED POLYMORPHIC DNA OF FOUR LOCAL PIG BREEDS IN SICHUAN PROVINCE

Huang Yongfu1,2, Zhang Yaping1, Zeng Fantong et al2   

  1. College of Animal Science and Technolegy, Sichuan Agricultural University/Yaan, Sichuan, China 625014
  • Received:1996-08-24 Online:1997-03-31 Published:2017-03-07

摘要: 使用21种随机引物,对四川省四个地方猪品种共18个个体进行了随机扩增多态DNA(RAPD)分析。结果表明:RAPD标记具有个体特异性,其多态总频率为78.4%;品种内遗传变异的大小顺序依次为荣昌猪、内江猪、雅南猪和成华猪;内江猪、雅南猪和成华猪间的亲缘关系较近,而荣昌猪与这三个品种的亲缘关系却较远。研究结果与这四个品种的地理分布状况和品种形成历史间存在一致性。

关键词: 随机扩增多态DNA, 遗传距离, 品系[49DA], 猪[49ME]

Abstract: In this study, random amplified polymorphic DNA(RAPD) analysis was used to estimate the diversity of four local pig breeds in Sichuan Province, i.e., Rongchang Pig, Neijiang Pig, Chenghua Pig and Yanan Pig. RAPD markers of 18 animals were detected with 21 arbitrary oligonucleotide primers, and the percentage of polymorphic fragments was 78.4%. According to the value of genetic diversity within breed, Rongchang Pig was the biggest, and Neijiang Pig was the second in order. In addition, there was a close genetic relationship between Neijiang Pig, Yanan Pig and Chenghua Pig, while Rongchang Pig had a far relationship with these three breeds. The results were consistent with the geographic distributions and breeding histories of these four breeds.

Key words: RANDOM AMPLIFIED POLYMORPHIC DNA, GENETIC DISTANCE, LINES(BREEDING), PIGS

中图分类号: 

  • S828.2