关键词: 外源DNA转导, 减压渗透法, 遗传变异, 电泳分析, 染色体, 水稻

Abstract: Sorghum, common wheat and millet were used as the donors of exogenous DNA and a rice cultivar Malai Red used as the recipient. The donors' DNAs were introduced into the recipient's embryos through decompressed permeation at the optimum stage for the embryo to receive exogenous DMA. Variations were observed in the generation of introduction. Isozyme analyses revealed different patterns among donors, recipient and transduced seedlings. Number and appearances of chromesomes in the root tip cells of the transduced plants at seedling and heading stages were the same as that in the root tip cells of recipient. More than ten variant lines were obtained.

Key words: EXOGENOUS DNA TRANSDUCTION, DECOMPRESSED PERMEATION, GENETIC VARIATION, ELECTROPHORESIS, CHROMOSOMES, PADDY