四川农业大学学报 ›› 1995, Vol. 13 ›› Issue (02): 144-149,253,254.doi: 10.16036/j.issn.1000-2650.1995.02.006

• 研究论文 • 上一篇    下一篇

鹅观草×大麦属间杂种的研究

周永红, 颜济, 杨俊良   

  1. 四川农业大学基础部、小麦所, 雅安市 625014
  • 收稿日期:1994-07-16 出版日期:1995-06-30 发布日期:2017-04-22
  • 基金资助:
    四川省科委应用基础资助项目

STUDY OF INTERGENERIC HYBRID BETWEEN ROEGNERIA TSUKUSHIENSIS VAR. TRANSIENS AND HORDEUM BULAGARE

Zhou Yonghong, et al   

  1. Department of Basic Sciences, Sichuan Agricultural University, Yaan, Sichuan, China 625014
  • Received:1994-07-16 Online:1995-06-30 Published:2017-04-22

摘要: 本文以分布于四川的鹅观草(Roegneria tsuknshiensis (Honda) B. R. Lu,Yen et J. L. Yang var. transiens (Hack.) B. R. Lu,Yan et J. L. Yang,2n=42,SSHHYYJ作母本,栽培二棱大麦(Hordeum vulgare L. cv. CA-5,2=14,II)作父本进行属间杂交,利用杂种幼胚组织培养技术,成功地获得了杂种F1.杂交结实率为6.58%,胚培成苗率为60.00%.杂种表现为多年生,具有很强的生活力,形态上表现为双亲的中间类型.F1自交不孕,用大麦回交亦不结实·杂种具有预期的2n=28(SHYI)条染色体。花粉母细胞减数分裂中期I平均形成27.24个单价体.0.20个棒状二价体,0.18个环形二价体,未发现多价体存在。表明鹅观草和大麦的染色体组间同源程度低。本文还探讨了大麦育种中利用鹅观草种质的可能性。

关键词: 鹅观草, 栽培二棱大麦, 属间杂交, 形态学, 细胞遗传学

Abstract: Roegneria tsukushicensis (Honda) B. R. Lu, Yen et J. L.Yang var. transiens (Hack.)B. R. Lu,Yen et J. L. Yang (2n=42,SSHHYY)in Sichuan as female parent was crossed with Hordeum vulgare. L. cv. CA-5 (2n-14, II). The intergeneric hybrids successfully obtained by means of embryo culture in vitro technique. The hybrid was perennial and vigorous, and it was morphologically similar to the middle type between two parents. 6.58% florets set seeds, and 60.00% embryos cultured developed into hybrid plants.All hybrid plants were self-sterile and set no seeds when backcrossed with barley.It indicated that the male in hybrid F1 was sterile.The hybrid expected chromosome numbers of 2n=28(SHYI).The mean meiotic configuration per PMC was 27.28+0.2II+0.16II. No multivalent was found. division at anaphase was abnormal,and unequal division and non-synchronized division were observed.The results indicated that the genomes between two parents existed a low affintiy.The possibility of utilizing Roegneria germplasm in barley breeding was discussed.

Key words: ROEGNERIA TSUKUSHIENSIS VAR. TRANSIENS, HORDEUM VULGARE, GENERIC CROSSES, MORPHOLOGY, CYTOGENETICS

中图分类号: 

  • S512.3