狂犬病(HEP/BHK21活疫苗的试制

doi:10.16036/j.issn.1000-2650.1994.02.024

四川农业大学学报 ›› 1994, Vol. 12 ›› Issue (02): 289-292.doi: 10.16036/j.issn.1000-2650.1994.02.024

狂犬病(HEP/BHK₂₁活疫苗的试制

郭万柱, 冯炳芳, 蒋文灿, 赵景芬, 庄冬跃, 谢跃, 余广海, 汪铭书

四川农业大学/动物科技学院, 雅安市 625014

收稿日期:1993-05-30 出版日期:1994-06-30 发布日期:2017-04-22

STUDIES ON THE PRODUCTION OF RABIES LIVE VACCINE (HEP/BHK₂₁)

Guo Wanzhu, et al

College of Animal Science and Technology Sichuan Agricultural University, Yaan, Sichuan, China 625014

Received:1993-05-30 Online:1994-06-30 Published:2017-04-22

摘要/Abstract

摘要： 狂犬病FluryHEP株经BHK₂₁细胞传代后,从1987年试制了7批疫苗,批间毒价稳定,MICLD₅₀均在10^-4.50/0.03ml以上,冻干苗毒价≥10^-4.50/0.03ml。冻干苗以5%蔗糖、1%明胶为保护剂,在-20℃保存一年,4-8℃保存一年,室温(18~25℃)保存7天,毒价均无明显下降。

关键词: 狂犬病(47MA), 冻干活疫菌, 抗体(47KD), 检测(52HB58BC)

Abstract: Seven lot of modified live virus rabies vaccine were prepared by growing HEP strain on BHk21 cell cultures from 1987 The titer of the vaccine was stable at different lot.A lyophilized live vaecine was prepared with 5% sucross and 15 glatine as stabilizer.The titer of this vaccine was usually ≥ 10-4.50 MICLD50/0.03ml, It was not significantly reduced after keeping the vaccine at-20℃ and 4-8℃ for one yeay, or at room temperature for 7 days.

Key words: RABIES, LYOPHILIZED LIVE VACCINE, ANTIBODIES, DETECTION

中图分类号:

S855.3

郭万柱, 冯炳芳, 蒋文灿, 赵景芬, 庄冬跃, 谢跃, 余广海, 汪铭书. 狂犬病(HEP/BHK₂₁活疫苗的试制[J]. 四川农业大学学报, 1994, 12(02): 289-292.

Guo Wanzhu, et al. STUDIES ON THE PRODUCTION OF RABIES LIVE VACCINE (HEP/BHK₂₁)[J]. jsau, 1994, 12(02): 289-292.

[1]	郭万柱, 陈代荣, 冯炳芳, 余广海, 蒋文灿, 罗时南, 龙平金, 王印. 狂犬病(HEP/BHK₂₁)活疫苗免疫期试验[J]. 四川农业大学学报, 1994, 12(02): 293-297.
[2]	郭万柱, 曹军, 孔树全, 冯炳芳, 蒋文灿. 应用ABC－ELISA和SPA－ELISA检测狂犬病疫苗免疫犬后血清中的抗体水平[J]. 四川农业大学学报, 1994, 12(02): 285-288.
[3]	郭万柱, 冯炳芳, 汪铭书, 蒋文灿. 狂犬病Flury HEP株的复制及其某些生物学性质的观察[J]. 四川农业大学学报, 1994, 12(02): 281-284.

狂犬病(HEP/BHK₂₁活疫苗的试制

STUDIES ON THE PRODUCTION OF RABIES LIVE VACCINE (HEP/BHK₂₁)

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