应用ABC－ELISA和SPA－ELISA检测狂犬病疫苗免疫犬后血清中的抗体水平

doi:10.16036/j.issn.1000-2650.1994.02.023

四川农业大学学报 ›› 1994, Vol. 12 ›› Issue (02): 285-288.doi: 10.16036/j.issn.1000-2650.1994.02.023

应用ABC－ELISA和SPA－ELISA检测狂犬病疫苗免疫犬后血清中的抗体水平

郭万柱, 曹军, 孔树全, 冯炳芳, 蒋文灿

四川农业大学动物科技学院, 雅安市 625014

收稿日期:1993-08-30 出版日期:1994-06-30 发布日期:2017-04-22

DETECTION OF ANTIBODIES FROM DOGS VACCINATED WITH RABIES VACCINE BY ABC-ELISA AND SPA-ELISA

Guo Wanzhu, et al

College of Animal Science and Technology Sichuan Agricultural University, Yaan, Sichuan, China 625014

Received:1993-08-30 Online:1994-06-30 Published:2017-04-22

摘要/Abstract

摘要： 本文报道应用ABC-ELISA和SPA-ELISA检测狂犬病疫苗免疫犬后血清中抗体的效价,比较研究了两种方法的特异性,敏感性和重复性,结果显示两种方法均适用于检测狂犬病血清抗体水平。ABC-ELISA较SPA-ELISA敏感性高4倍,前者的阳性检出率(100%)高于后者(92.8%)。

关键词: 狂犬病(47MA), 抗体(47KD), 检测(52HB58BC)

Abstract: This paper reported detection of antibodies from dogs vaccinated with rabies vaccine by ABC-ELISA and SPA-ELISA, Thespecifiaty, sensitivity and stability of both mothods were compared.The results showed that both methods were applicable todetccting the level of antibody to rabies virus.The sensitivity of ABC-ELISA was four times as much as that of SPA-ELSA.Thepositive rate (100%) in ABC-ELISA was higher than that (92.8%) in SPA-ELISA.

Key words: RABIES, ANTIBODIES, DETECTION

中图分类号:

S855.3

郭万柱, 曹军, 孔树全, 冯炳芳, 蒋文灿. 应用ABC－ELISA和SPA－ELISA检测狂犬病疫苗免疫犬后血清中的抗体水平[J]. 四川农业大学学报, 1994, 12(02): 285-288.

Guo Wanzhu, et al. DETECTION OF ANTIBODIES FROM DOGS VACCINATED WITH RABIES VACCINE BY ABC-ELISA AND SPA-ELISA[J]. jsau, 1994, 12(02): 285-288.

[1]	郭万柱, 陈代荣, 冯炳芳, 余广海, 蒋文灿, 罗时南, 龙平金, 王印. 狂犬病(HEP/BHK₂₁)活疫苗免疫期试验[J]. 四川农业大学学报, 1994, 12(02): 293-297.
[2]	郭万柱, 冯炳芳, 蒋文灿, 赵景芬, 庄冬跃, 谢跃, 余广海, 汪铭书. 狂犬病(HEP/BHK₂₁活疫苗的试制[J]. 四川农业大学学报, 1994, 12(02): 289-292.
[3]	郭万柱, 冯炳芳, 汪铭书, 蒋文灿. 狂犬病Flury HEP株的复制及其某些生物学性质的观察[J]. 四川农业大学学报, 1994, 12(02): 281-284.

应用ABC－ELISA和SPA－ELISA检测狂犬病疫苗免疫犬后血清中的抗体水平

DETECTION OF ANTIBODIES FROM DOGS VACCINATED WITH RABIES VACCINE BY ABC-ELISA AND SPA-ELISA

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