关键词: 瓜蒌瓤, L-酪氨酸, 酪氨酸酶, 抑制作用

Abstract: [Objective] The aim of the study was to explore the inhibiting effect of extract from fruit pulp of T. kirilowii on tyrosinase activity. [Method] In this study,microculture plate reader was used to investigate the effects of extraction solvent,L-tyrosine volume,the amount of tyrosinase and reaction time on tyrosinase activity. [Results] When 50 μL L-tyrosine with 0.362 mg/mL and 50 μL tyrosinase with 327 U/mL were added,and the reaction time was 5 min,the inhibiting effect of extract from fruit pulp of T. kirilowii on tyrosinase activity were the most significant. [Conclusion] The extract from fruit pulp of T. kirilowii had significant inhibiting effects on tyrosinase activity.Therefore,it can be used as a natural whitening cosmetic additive.

Key words: fruit pulp of Trichosanthes kirilowii Maxim., L-tyrosine, tyrosinase, inhibition