关键词: 猪, microRNA, 背最长肌, 腰大肌, 高通量测序

Abstract: [Objective] This experiment was conducted to investigate the effects of microRNAs on the development of porcine skeletal muscle.[Method] Illumina Solexa sequencing technology were applied to determine the miRNA expression patterns between porcine longissimus doris muscle and Psoas major muscle.[Results] A total of 15.22 M and 17.52 M mappable sequences were expressed in longissimus doris muscle and Psoas major muscle library respectively.Totally 695 unique miRNAs were observed,and 363 were co-expressed in two libraries,of which,193 were significantly differentially expressed between longissimus doris muscle and and Psoas major muscle library (P<0.01).[Conclusion] All these differences indicated the essential roles of miRNAs in the phenotypic variations of different muscle fiber types.

Key words: pig, microRNA, longissimus doris muscle, Psoas major muscle, deep sequencing