四川农业大学学报 ›› 2011, Vol. 29 ›› Issue (02): 147-153.

• 研究论文 • 上一篇    下一篇

利用小麦四倍体重组自交系群体定位穗部相关性状QTL

严俊1,张玲玲1,万兵1,苟君波1,王运长1,许昌敏1,Tzion Fahima1,程剑平2   

  1. 1.
    2. 贵州大学
  • 收稿日期:2011-02-27 修回日期:2011-04-11 出版日期:2011-06-30 发布日期:2011-06-30
  • 通讯作者: 程剑平
  • 基金资助:

    贵州省优秀青年科技人才培养计划;贵州省“十一五”重点攻关项目

QTL mapping analysis of spike related traits in durum wheat × wild emmer wheat RIL population

  • Received:2011-02-27 Revised:2011-04-11 Online:2011-06-30 Published:2011-06-30
  • Contact: Cheng JianPing

摘要:

利用种植于黔中的来自以色列Gitit的野生二粒小麦(编号:G18-16)与来自欧洲的四倍体栽培小麦Langdon (Ldn)杂交构建的重组自交系 F6代152个家系,进行了抽穗期、单株有效穗数、穗粒数、穗长、芒长等数量性状基因位点(QTL)定位与分析,发现全部家系在五个性状上表现出宽广的遗传差异。14个穗部相关性状加性QTL被定位,LOD值为1.9~13.4,贡献率为7.3~54.2%。控制抽穗期的QTL共3个,定位在3A(2个)和7B上;在2A (2个) 和5A上共找到3个控制穗粒数的QTL;在5B上找到2个控制有效穗数的QTL;控制穗长的3个QTL分布在5A(2个)和7A上;在4B, 5A和7A上共找到3个控制芒长的QTL。获得的QTL可用于分子标记辅助育种改良现代栽培小麦。

关键词: 野生二粒小麦, 抽穗期, 单株有效穗数, 穗粒数;穗长, 芒长, 数量性状基因位点(QTL)

Abstract:

Wild emmer wheat, Triticum dicoccoides, is the progenitor of modern tetraploid and hexaploid cultivated wheats, which genepool harbor a rich allelic repertoire for various traits. In this paper, the genetic and phonotypic basis of spike traits, e.g. heading stage (HS), spike number per plant (SPP), kernel number per spike (KNS), spike length (SL), and awn length (AL) were studied in tetraploid wheat population consists of 152 F6 recombinant inbreed lines (RILs) derived from a cross between T. dicoccoides (acc# G18-16) and durum wheat cultivar (Langdon) grown in the central region of Guizhou Province, China. Wide genetic variation was found among RILs for all studied traits. A total of 14 QTL effects associated with five spike related traits were detected, with a LOD score range of ca.1.9 to 13.4. The identified QTLs included: three QTLs for HS located on chromosomes 3A (2 QTLs) and 7B, explaining phenotypic variation 54.2 and 9.4%; three QTLs for KNS, located on chromosomes 2A (2 QTLs) and 5A, explaining 14.6 and 24.3 of phenotypic variation; two QTLs for SPP, located on chromosome 5B (2 QTLs), explaining 44.7% of phenotypic variation; three QTLs for SL, located chromosomes 5A (2 QTLs) and 7A,explaining 54.2 and 9.4 of phenotypic variation; three QTLs for AL, located chromosomes 4B, 5A and 7A, explaining 7.3, 7.8 and 9.8 of phenotypic variation, respectively. The identified QTLs could be useful in future marker-assisted selection programs, facilitating the use of wild alleles for yield enhancement in elite wheat cultivars.

Key words: Triticum dicoccoides, Heading stage; Spike number per plant; Kernel number per spike; Spike length; Awn length; Quantitative trait locus (QTL)