关键词: 白僵菌, 液体发酵, 分生孢子, 生物防治

Abstract: Preservative effects of two preservatives on the liquid preparation of Beauveria bassiana were also tested. The virulence of liquid preparation of B. bassiana to Dendrolimus Punctatus Walker was explored. The experiment shows that 2% glycerine(an ideal preservatative) preserves surface of liquid from appearing mycelial membrance, and maintains the high spore germination of B. bassiana for 45~60 days under 25℃ while physiological slat has no effective protection on the liquid preparation. The liquid preparation is the same as the powder of B. bassiana in both the pathogenetic rate and general death of Dendrolimus Punctatus Walker. The death of the insect by respectively spraying of two preparations all begins to appear in the 9^th day, and then reaches peak in the 12^th day. The result further illustrates the possibility of applying the liquid preparation of B. bassiana.

Key words: BEAUVERIA BASSIANA, LIQUID FERMENTATION, CONIDIA, BIOLOGICAL CONTROL