关键词: 普通小麦, 硬粒小麦-簇毛麦双二倍体, 杂种, 染色体配对, Giemsa-C技术

Abstract: The Meiotic behavior of T. durum-D. villosum amphiploid with three bread wheat varieties (lines) was employed by acetocarmine and C-banding staining of PMCs at first metaphase to reveal homoeologous pairing between wheat and D. villosum chromosomes. The results indicated that the chromosome pairings more than expected in hybrid cell were homoeologous pairings either of wheat-D. villosum chromosomes or of wheat wheat chromosomes. On the other hand, the pairings less than expected in a hybrid cell should be considered as homologous pairings of A-A or B-B chromosomes according to traditional Feulgen stained method, in fact, a few bivalents between wheat and D. villosum chromosomes in those cell with bivalents less than 14 were also observed, which reflected that estimation of homoeologous pairing between wheat and alien species genome chromosomes by standard Feulgen staining technique in AABBDV hybrid was non-accurate. The difficulties to obtain wheat lines which carried desirable genes from D. villosum through homoeologous pairing and recombination were also discussed.

Key words: COMMON WHEAT, T. DURUM-D. VILLOSUM AMPHIPLOID, HYBRID, CHROMOSOME PAIRING, GIEMSA-C BANDING TECHNIQUE