关键词: 水牛, 毛, 妊娠诊断, 孕甾酮

Abstract: Preliminary study on early pregnancy diagnosis in 10 buffaloes (Murrah x Local swamp buff, F₁) based on hair progesterone determination by means of a competitive protein-binding assay has been conducted. Measurements of progesterone profile during oestrus cycle showed that the difference in hair progesterone content between pregnant and non-pregnant buffaloes became significant (p<0.05) and highly significant (p<0.01) during days 16-18 and 20-24 post-oestrus respectively. When hair samples were taken at the back of 17 buffalloes and on the 24th day after oestrus, the accuracy of pregnancy diagnosis was 88.8% and 100% for pregnant and non-pregnant animals respectively as compared with the results obtained from rectal palpation two months after insemination, the criterion for discriminating pregnancy and non-pregnancy was taken as that:higher than 4.5ng/100mg hair, pregnancy, lower than 3.5ng/100mg hair, non-pregnancy.

Key words: BUFFALO, HAIR, CYESIOGNOSIS, PROGESTERONE