四川农业大学学报 ›› 2013, Vol. 31 ›› Issue (04): 414-418.doi: 10.3969/j.issn.1000-2650.2013.04.010

• • 上一篇    下一篇

道地产区金银花的ISSR遗传多样性分析

韩琳娜1, 康玉秋1,2, 郭庆梅1, 周凤琴1, 张永清1   

  1. 1. 山东中医药大学药学院, 济南 250355;
    2. 扬子江药业集团上海海尼药业有限公司, 上海 201318
  • 收稿日期:2013-10-11 出版日期:2013-12-31 发布日期:2017-02-28
  • 作者简介:韩琳娜,讲师,博士,主要从事分子生药学的研究,E-mail:linnahan@163.com。
  • 基金资助:
    十二五"国家科技发展计划(2011BAI06B01,金银花规范化种植基地优化升级及系列产品综合开发研究);国家自然科学基金(30973879,不同种质忍冬AFLP指纹图谱及其药材组分HPLC指纹图谱关联性的研究)

ISSR-based Genetic Diversity of Lonicera japonica in Authetic Region

HAN Lin-na1, KANG Yu-qiu1,2, GUO Qing-mei1, ZHOU Feng-qin1, ZHANG Yong-qing1   

  1. 1. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;
    2. Yangtze River Pharmaceutical Group, Shanghai 201318, China
  • Received:2013-10-11 Online:2013-12-31 Published:2017-02-28
  • Contact: 郭庆梅,教授,博士,主要从事中药质量控制与资源的研究,E-mail:qmguo@sina.com。 E-mail:qmguo@sina.com

摘要: [目的] 对三大道地产区的金银花品种进行遗传多样性分析,为道地产区种质资源利用与开发提供理论依据。[方法] 应用ISSR分子标记技术对27个金银花原植物种质资源进行遗传多态性、相似性和聚类分析。[结果] 筛选出16个多态性引物,获得344条带,多态性比率为88.4%;遗传相似系数在0.50~0.90之间;聚类分析表明,27个品种被分为2个类群。[结论] ISSR标记可以应用于金银花样品间遗传多样性分析;道地产区的金银花种内不同地域或不同植物来源的材料间存在较大遗传差异。

关键词: 金银花, ISSR, 遗传多样性, 聚类分析

Abstract: [Objective] The objective of this study was to detect the genetic diversity of L. japonica from three authentic regions and to provide the theoretical basis for development and utilization of germplasm resources.[Method] Intersimple sequence repeat (ISSR) was used to analyze genetic polymorphism,similarity and cluster map of 27honeysuckle germplasm resources.[Results] 344 bands were obtained from 16selected primers.The percentage of polymorphic bands was 88.4%. The genetic similarity varied from 0.50to 0.90.27varieties were divided into 2groups by cluster analysis.[Conclusion] ISSR markers can be applied for genetic diversity of L. japonica.There were obvious genetic differences among regions and plant sources.

Key words: Lonicera japonica, ISSR, genetic diversity, cluster analysis

中图分类号: 

  • R282.71