关键词: 猪, 皮下脂肪, 内脏脂肪, 脂肪酸组成, 炎症, RNA-Seq

Abstract: [Objective] The purpose of the present research was to explore the differences in metabolism and transcripts between porcine subcutaneous and visceral adipose tissues.[Method] A phenotype analysis and a comprehensive transcriptome analysis by high-throughput RNA-Seq was carried out in three porcine adipose tissues,including one subcutaneous adipose tissue (backfat) and two visceral adipose tissues (greater omentum and mesenteric adipose tissue).[Results] Visceral adipose tissues had smaller adipocyte volume and showed significantly higher content of Saturated Fatty Acid (P<0.01) but lower content of Unsaturated Fatty Acids (P<0.05) than subcutaneous adipose tissue,meanwhile,we obtained 30 532 913-33 479 707 unique mapped reads that covered 80.2% of the current annotated transcripts and identified 4 583-4 765 novel transcript units,and further identified 936-1 029 splicing genes,16 559 SNPs and 1 481 INDELs.We also identified 183 genes that differently expressed between the subcutaneous and visceral adipose tissues,which were mainly associated with the biological categories of the unsaturated fatty acid biosynthetic,carboxylic acidmetabolic and inflammatory response.[Conclusion] This study provided a global view of transcriptome in porcine adipose tissues,and the differential gene expression analysis showed some potential genes that could partly revel the function characteristics and differences between these two types of adipose tissue.

Key words: pig, subcutaneous adipose tissue, visceral adipose tissue, fatty acid composition, inflammatory response, RNA-Seq