关键词: 披碱草属, 扩增片段长度多态性标记(AFLP), 银染, 体系优化

Abstract: The key factors affecting the AFLP analysis in Elymus were optimized,including the digesting time and the content of the EcoRⅠ and MseⅠ,diluted ratio and concentrations of primers in the pre-amplification and selective amplification.Furthermore,the methods of Bassam and Sanguinetti silver staining were also compared in the staining quality.The optimal reaction system of AFLP were established,which including digest with 3 units of each enzymes at 37℃ for 3 h,pre-amplification performed with templates diluted 10 times and 1.5 ng/μL primers,selective amplification performed with templates diluted 20 times and 2 ng/μL primers,as well as stained with Bassam silver staining.The results could apply for the further study on AFLP in Elymus.

Key words: Elymus, AFLP, silver staining, optimization