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四川农业大学学报 ›› 2016, Vol. 34 ›› Issue (01): 54-59.doi: 10.16036/j.issn.1000-2650.2016.01.010

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云南峨毛茶亲缘关系的ISSR分析及指纹图谱构建

吴田1, 蓝增全2   

  1. 1. 西南林业大学园林学院, 昆明 650224;
    2. 西南林业大学环境科学与工程学院, 昆明 650224
  • 收稿日期:2015-11-04 发布日期:2016-02-29
  • 通讯作者: 蓝增全,教授,硕士,主要从事茶学的研究,E-mail:461257271@qq.com。 E-mail:461257271@qq.com
  • 作者简介:吴田,副教授,博士,主要研究方向为植物分子生物学,E-mail:2351417655@qq.com。
  • 基金资助:
    国家星火计划(金花茶种苗繁育与茶膏加工技术的示范推广2014GA830016);2015年三区人才支持计划(21501737)

ISSR Analysis on the Relationship and Construction of Fingerprint in Emao Teas of Yunnan

WU Tian1, LAN Zeng-quan2   

  1. 1. Landscape Architecture College, Southwest Forestry University, Kunming 650224, China;
    2. Environment Science and Engineering College, Southwest Forestry University, Kunming 650224, China
  • Received:2015-11-04 Published:2016-02-29

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摘要： [目的] 了解和保护峨毛茶大茶树资源。[方法] 采用ISSR技术对云南峨毛地区的5份茶样资源的遗传多样性和亲缘关系进行研究。[结果] 结果表明:5份峨毛茶样共扩增出94条清晰的DNA条带,其中多态性带73条,占77%,遗传相似系数介于0.45~0.64之间,表明来自同一地区的峨毛茶之间已经产生遗传分化。[结论] 利用823引物构建了5份峨毛茶的指纹图谱,为今后峨毛茶的鉴定提供了参考。

关键词: 峨毛茶, 指纹图谱, 遗传多样性

Abstract: [Objective] The aim of the study was to know and protect the large tree resources of Emao teas. [Method] The genetic diversity and genetic relationship of five Emao tea samples from Yunnan were analyzed by ISSR technology. [Results] The results showed that 94 clear DNA bands in Emao tea were obtained through amplification. 73 bands of them were polymorphic and the average polymorphism rate was 77%. The genetic similarity coefficient was between 0.45 and 0.64, indicating that there was genetic diversity in Emao teas. [Conclusion] The fingerprint of five teas was constructed using the ISSR primer of 823, which can provide a molecular biology method for the identification of Emao teas.

Key words: Emao tea, fingerprint, genetic diversity

中图分类号: 

  • R282.71
ISSN 1000-2650
CN 51-1281/S
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