关键词: 猪传染性胃肠炎病毒, SC-H株, M基因, 克隆, 序列分析

Abstract: A pair of primers for M gene was designed according to the published cDNA sequence of transmissible gastroenteritis virus (TGEV)on Genbank,which was used to amplify the complete cDNA fragments of M gene by reverse transcription polymerase chain reaction (RT-PCR).The amplified fragments were cloned into pMD18-T vector and the recombinant plasmid pMD-M was identified by restriction enzyme analysis and sequencing,the nucleotide sequences and deduced amino acid sequences were analyzed in comparison with some other published TGEV strains.The results show the amplified fragments were 852 bp,the M gene was 789 bp and coding 262 amino acids.Compared with the strains Purdue,TO14,TS,HN2002,FS772/70,TFI and 96-1933,the homology of nucleotide sequences was 97.8%,94.2%,97.2%,97.8%,99.7%,96.8% and 98.2% respectively,the homology of nucleotide sequences was 96.6%,92.0%,95.4%,96.6%,99.2%,95.8% and 97.7% respectively,which show that M gene of different TGEV strains was highly conservative.The phylogenetic analysis show SC-H strains has a nearest genetic relationship with Strain Prude.

Key words: transmissible gastroenteritis virus, SC-H, M gene, cloning, sequence analysis