四川农业大学学报 ›› 2008, Vol. 26 ›› Issue (01): 45-47.doi: 10.16036/j.issn.1000-2650.2008.01.014

• 研究论文 • 上一篇    下一篇

雄黄兰组织培养和快繁技术研究

刘正兰, 刘军   

  1. 四川农业大学 林学园艺学院, 四川 雅安 625014
  • 收稿日期:2007-09-29 出版日期:2008-03-31 发布日期:2017-02-28

Study on Tissue Culture and Rapid Propagation Techniques in Crocosmia crocosmifolra

LIU Zheng-lan, LIU Jun   

  1. College of Forestry and Horticulture, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2007-09-29 Online:2008-03-31 Published:2017-02-28

摘要: 以雄黄兰的球茎为外植体,进行组织培养和快速繁殖技术研究。结果表明,芽诱导的最佳培养基为MS培养基添加6-BA3.0mg/L和NAA0.1mg/L。芽增殖的最佳培养基为MS添加6-BA1.0mg/L和NAA0.3mg/L,增殖系数大于7。生根培养基用添加IBA0.5mg/L的1/2MS培养基为宜,生根率达73.0%以上。

关键词: 雄黄兰, 组织培养, 快繁技术

Abstract: The tissue culture and rapid propagation techniques were developed by using corm as explant in Crocosmia crocosmifolra. The results indicated that the optimal medium for bud inducement is MS medium with 6-BA 3.0 mg/L and NAA 0.1 mg/L added. The optimal medium for bud multiplication is MS medium with 6-BA 1.0 mg/L and NAA 0.3 mg/L added. The multiplication rate is higher than 7. The proper rooting medium is 1/2 MS medium with IBA 0.5 mg/L added. The rooting rate is up to 73.00%.

Key words: Crocosmia crocosmifolra, tissue culture, rapid propagation technique

中图分类号: 

  • S682.2