关键词: 猪圆环病毒, 全基因, 克隆, 序列分析

Abstract: One pair of primers was designed based on the Genbank published sequence of Porcine Circovirustype 1(PCV1) to amplify the complete PCV1 genome of YA1 strain, resulting a 1759 bp PCR product.Then cloned the PCR product into pMD18-T vector to obtain the recombinant vector PMD18-T-PCV1-YA.Enzyme and sequencing analysis shows that the complete sequence of PCV1 was successfully cloned.Sequenced similarity analysis show ed that PCV1-YA1 strain shared 98.8%~99.9% nucleotide sequence identity with the other six strains in GenBank.Complete PCV2(1767 bp) sequence of SC strain was cloned into PMD18-T-PCV2-SC successfully using the same method, and sequenced similarity analysis showed that PCV2-SC strain shared 93.6%~98.6% nucleo tide sequence identity with other strains in GenBank.The nucleotide sequence identity between PCV1-YA1 and PCV2-SC is only 69.2%.The two major open-reading frame analysis shows that the nucleotide sequence identity of ORF1 and ORF2 between PCV1-YA1 and PCV2-SC are 85.3% and 66%, and their deduction amino acids identity are 85.3% and 65.2% respectively.The hydrophobic prediction revealed that PCV1 ORF1 is similar to PCV2 ORF1, and the transmembrane prediction revealed ORF2 are difference between the two strains.

Key words: porcine circovirus, com plete genome, clone, sequenced similarity analysis