四川农业大学学报 ›› 2006, Vol. 24 ›› Issue (04): 369-374.doi: 10.16036/j.issn.1000-2650.2006.04.001

• 研究论文 •    下一篇

仲彬草属植物ITS序列PCR产物直接测序与克隆测序比较分析

曾建, 张利, 凡星, 张春, 张海琴, 周永红   

  1. 四川农业大学 小麦研究所, 四川 都江堰 611830
  • 收稿日期:2006-09-04 出版日期:2006-12-31 发布日期:2017-03-06
  • 基金资助:
    国家自然科学基金项目(编号:30470135和30270099);教育部长江学者和创新团队发展计划(编号:IRT0453)

Comparison Analysis on Direct Sequencing of PCR Products and Sequencing of Cloning PCR Products of ITS Sequence of Nuclear rDNA in Kengyilia(Poaceae: Triticeae)

ZENG Jian, ZHANG Li, FAN Xing, ZHANG Chun, ZHANG Hai-qin, ZHOU Yong-hong   

  1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China
  • Received:2006-09-04 Online:2006-12-31 Published:2017-03-06

摘要: 通过PCR产物克隆测序和直接产物测序的方法对禾本科小麦族仲彬草属10个物种的rDNA内转录间隔区(ITS)及5.8S rDNA进行了序列测定和系统发育分析。结果表明:该属植物ITS区直接产物测序与克隆测序存在较大差异,克隆测序序列反映的系统发育关系与形态学、地理分布、细胞学、分子标记分析结果基本一致。因此,采用克隆测序的方法对仲彬草属植物系统发育分析比直接测序方法更为可靠。

关键词: 仲彬草属, ITS序列, 序列测定, 系统发育分析

Abstract: The results of direct sequencing of PCR products and sequencing by cloning PCR products of the internal transcribed spacers and the 5.8S coding regions of nuclear ribosomal DNA in Kengyilia were compared and phylogenetically analyzed. The results show that ITS sequences are of existed differences between the direct sequencing of PCR products and sequencing by cloning PCR products. The phylogenetic relationship in Kengyilia species based on sequences by cloning PCR products is consistent with the analysis of morphology, geographical distribution, cytology and molecular markers. Therefore, phylogenetic analysis carried out by sequencing of cloning PCR products is more credible than that inferred from direct sequencing of PCR products.

Key words: Kengyilia, ITS sequences, sequencing, phylogenetic analysis

中图分类号: 

  • Q949.714