四川农业大学学报 ›› 2006, Vol. 24 ›› Issue (03): 344-347.doi: 10.16036/j.issn.1000-2650.2006.03.021

• 研究简报 • 上一篇    下一篇

令箭荷花组织培养与植株再生的研究

刘帆, 倪苏, 田孟良   

  1. 四川农业大学 农学院, 四川 雅安 625014
  • 收稿日期:2006-04-17 出版日期:2006-09-30 发布日期:2017-03-06
  • 基金资助:
    四川农业大学农学院基金资助

Study on Tissue Culture and Plant Regeneration of Nopalxochia ackermannii

LIU Fan, NI Su, TIAN Meng-liang   

  1. College of Agriculture, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2006-04-17 Online:2006-09-30 Published:2017-03-06

摘要: 采用令箭荷花植株顶端幼嫩茎段作为外植体,以MS为基本培养基,采用不同激素配比分别诱导获得了愈伤组织、丛生芽和根。筛选出了适合令箭荷花植株再生的最佳激素浓度配比:6-BA 2 mg/L+NAA 0.2 mg/L适合愈伤组织的诱导,6-BA 1 mg/L+NAA 0.1 mg/L适合诱导丛生芽,IBA 0.2 mg/L有利于根的分化。

关键词: 令箭荷花, 组织培养, 植株再生

Abstract: The top tender stalks of Nopalxochia ackermannii are used as explants in this paper. On the base of MS medium which are added different hormones, then the callus, adventitious buds and roots are induced respectively. The best density of hormones is selected from many kinds of combinations, in which the best one for inducing callus, buds and root are 6-BA 2 mg/L+NAA 0.2 mg/L, 6-BA 1 mg/L+NAA 0.1 mg/L and IBA 0.2 mg/L, respectively. The high efficient regeneration system of in vitro culture of Nopalxochia ackermannii is also established.

Key words: Nopalxochia ackermanni, tissue culture, plant regeneration

中图分类号: 

  • S682