四川农业大学学报 ›› 2006, Vol. 24 ›› Issue (03): 247-251.doi: 10.16036/j.issn.1000-2650.2006.03.003

• 研究论文 • 上一篇    下一篇

野生二粒小麦α-淀粉酶抑制因子编码基因序列分析

江斌1, 王际睿1, 魏育明1,2, 颜泽洪1,2, 郑有良1,2   

  1. 1. 四川农业大学 小麦研究所, 四川 都江堰 611830;
    2. 四川农业大学 西南作物基因资源与遗传改良教育部重点实验室, 雅安 625014
  • 收稿日期:2006-02-28 出版日期:2006-09-30 发布日期:2017-03-06
  • 基金资助:
    国家自然科学基金(No.30571163);高等学校全国优秀博士学位论文作者专项资金(No.200357和No.200458);长江学者和创新团队发展计划(No.IRT0453)

Sequence Analysis of α-amylase Inhibitors Genes from Triticum dicoccoides

JIANG Bin1, WANG Ji-rui1, WEI Yu-ming1,2, YAN Ze-hong1,2, ZHENG You-liang1,2   

  1. 1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China;
    2. Ministry of Education Key Laboratory for Crop Genetic Resources and Improvementin Southwest China, Sichuan Agricultural University, Yaan 625014, China
  • Received:2006-02-28 Online:2006-09-30 Published:2017-03-06

摘要: 对3份野生二粒小麦(Triticum dicoccoides)材料的抗虫24 kDaα-淀粉酶抑制因子编码基因进行分离克隆,得到72个24 kDaα-淀粉酶抑制因子基因,并进行序列分析。结果发现,α-淀粉酶抑制因子在野生二粒小麦中以多拷贝形式存在,经卡方检验初步推断野生二粒小麦中的α-淀粉酶抑制因子成熟蛋白编码基因序列变异类型在供试材料间无显著差异。序列分析表明,24 kDaα-淀粉酶抑制因子成熟蛋白编码基因序列无论在野生二粒小麦种内还是与小麦属的其他物种之间,都具有很高的一致性,说明该基因在进化过程中十分保守。

关键词: 野生二粒小麦, α-淀粉酶抑制因子, 序列分析

Abstract: Seventy-two genes encoding the 24kDa α-amylase inhibitors from three genotypes of T. dicoccoides were characterized. According to sequences alignment, it is found that the 24kDa α-amylase inhibitors in T. dicoccoides are encoded by multi-gene. The distribution of inhibitor forms has no distinct difference among the three accessions of T. dicoccoides by χ2 test. The nucleotide sequences of 24 kDa α-amylase inhibitors are with high coherence between T. dicoccoides and related species, which indicates that the α-amylase inhibitors in this family are conserved in evolution.

Key words: T. dicoccoides, α-amylase inhibitor, sequence analysis

中图分类号: 

  • S512.1032