四川农业大学学报 ›› 2006, Vol. 24 ›› Issue (02): 161-165.doi: 10.16036/j.issn.1000-2650.2006.02.009

• 研究论文 • 上一篇    下一篇

油菜根肿病菌形态及生物学特性研究

马淑青1, 黄云1, 王铮一2, 赵艳琴1, 曾富春1   

  1. 1. 四川农业大学 农学院, 四川 雅安 625014;
    2. 郫县农业局植保站, 四川 郫县 611730
  • 收稿日期:2006-02-24 出版日期:2006-06-30 发布日期:2017-03-06

Modality and Biological Characteristic of Plasmodiophora brassicae in Rape

MA Shu-qing1, HUANG Yun1, WANG Zheng-yi2, ZHAO Yan-qin1, ZENG Fu-chun1   

  1. 1. College of Agriculture, Sichuan Agricultural University, Yaan 625014, Sichuan, China;
    2. Plant Protection Station of Pixian Country Agriculture Department, Pixian 611730, Sichuan, China
  • Received:2006-02-24 Online:2006-06-30 Published:2017-03-06

摘要: 油菜根肿病近年在四川省发生日趋严重。本文报道了油菜根肿病菌休眠孢子的显微形态结构,其在扫描电镜下休眠孢子近球形,孢壁不光滑,有乳状突起,直径为1.9~4.3μm(平均3.5μm);透射电镜下游动孢子近球形、肾形或椭圆形,直径为1.6~3.5μm(平均2.8μm),同侧着生不等长尾鞭式双鞭毛。观察了休眠孢子萌发形成游动孢子、休止孢、休止孢再萌发形成芽管的过程。病菌休眠孢子萌发的最适温度是24℃,最适pH值是6.3,腐烂处理促进休眠孢子萌发,光抑制休眠孢子萌发,休眠孢子在过滤灭菌的根分泌物中萌发率最高为62.50%。

关键词: 油菜, 根肿菌, 休眠孢子, 形态, 生物学特性

Abstract: Recently, the incidence of crucifer clubroot in rape is gradually increases. This text reports the modality of resting spores of crucifer clubroot in rape. The resting spores are nearly spherical, not slippery and mamillated on the wall in scanning electron microscope, their diameters range from 1.9 to 4.3 μm, and the mean is 3.5 μm. The primary zoospores with unequal length biflagellates are in nearly spherical, or nephroid, or oval shape in transmission electron microscope, and their size varies widely from 1.6 to 3.5 μm, and the mean is 2.8 μm. It is observed that the changing course of the resting spores turn into the primary zoospores, the silent spores in order, and then one silent spore germinates out a tubal structure. The optimum temperature for the germination of resting spores is 24 ℃, the optimum pH is 6.3. Treatment of rotted clubs is helpful to enhance the germinated rate. The visible light inhibit the germination of the resting spores. The highest germinated rate for the resting spores is 62.50% in the root exudates. The results show that the conditions for the germination of resting spores are accordant with the disease's generant condictions.

Key words: rape, Plasmodiophora brassicae, resting spore, modality, biological characteristic

中图分类号: 

  • S565.4