关键词: 过氧化物同工酶, 葡萄品种, 霜霉病抗性, 鉴定

Abstract: The peroxidase isozyme of 22 grape varieties are analyzed by plumb plate of polyarclamide gel electrophoresis. The results of electrophoresis are 6 bands of peroxidase and 17 zymograms types, which indicates that POD isozyme cannot identify 22 grape varieties and each POD isozyme has 3 to 5 bands; the band of 0.6089 Rf is characteristic of 22 grape varieties, the band of 0.3333 Rf is the featuring Rf of Downy mildew variety, the bands of 0.2661 Rf, 0.2978 Rf and 0.4455 Rf are the featuring Rf of anti-Downy mildew variety. According to the results of SPSS and UPGMA cluster analysis, the grape varieties can be divided into five groups:grope 1 and 2 are 12 Vitis uinifear such as Centennial seedless, Wuhe 8611 etc showing susceptibility and mid-susceptibility to downy mildew. Group 3, 4, 5 are 10 Vitis uninifear and V.labrussca hybrid such as Fujiminori, Fanganghei, Kyoho, showing resistance mid-resistance to Downy mildew. This result is the same as the appraisal result in field natural and indoors inoculation. Therefore, the featuring Rf of POD isozyme is applicable to identifying disease reaction to downy mildew type of grape varieties, but not effective in the identification of grape varieties.

Key words: peroxidase isozyme, grape varieties, resistance to downy mildew, identification