四川农业大学学报 ›› 2005, Vol. 23 ›› Issue (02): 137-141.doi: 10.16036/j.issn.1000-2650.2005.02.001

• 研究论文 •    下一篇

东方小麦高分子量谷蛋白亚基组成分析

徐黎黎, 颜泽洪, 魏育明, 郑有良   

  1. 四川农业大学 小麦研究所, 四川 都江堰 611830
  • 收稿日期:2005-04-01 出版日期:2005-06-30 发布日期:2017-04-21
  • 基金资助:
    高等学校全国优秀博士学位论文作者专项资金(200357)。

Analysis of HMW Glutenin Subunits in Triticum turanicum Jakubz.

XU Li-li, YAN Ze-hong, WEI Yu-ming, ZHENG You-liang   

  1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China
  • Received:2005-04-01 Online:2005-06-30 Published:2017-04-21

摘要: 采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)方法,对87份东方小麦(Triticum turanicum Jakubz.)的高分子量谷蛋白亚基(HMW-GS)组成检测表明,Glu-A1和Glu-B1分别具有2种和5种不同的亚基类型。在Glu-B1位点上,东方小麦具有其明显的特点,13+16亚基出现频率最高,达60.92%,而这种亚基在普通小麦中属稀有亚基。在Glu-A1位点上,东方小麦与普通小麦、密穗小麦和硬粒小麦相似,null为主要亚基类型。筛选出具有2*或7+8优质亚基的材料各9份,其中来自埃塞俄比亚的1份材料CItr 14598具有(2*,7+8)优质亚基组合,确属品质育种中难得的优异基因资源,可供普通小麦优质育种利用。

关键词: 东方小麦, 高分子量谷蛋白亚基(HMW-GS), 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)

Abstract: The high-molecular-weight glutenin subunits (HMW-GS) in eighty-seven Triticum turanicum Jakubz. accessions are analyzed by SDS-PAGE. Seven alleles are identified, with two at Glu-A1 and five at Glu-B1 locus. At the Glu-B1 locus, the frequence of subunits 13+16 is relatively high (60.92%), whereas in common wheat, this subunits is very rare, which indicates that HMW-GS composition of T.turanicum differs obviously from common wheat. The variation of Glu-A1 of T.turanicum was similar to common wheat, compactum wheat and durum wheat, and null subunit is the most common allele at Glu-A1. Nine T.turanicum accessions with 2* or 7+8 subunits are identified respectively. CItr 14598 from Ethiopia with superb HMW subunits combination (2*, 7+8) is a scarce desirable gene resource and it could be used in the improvement of wheat quality.

Key words: Triticum turanicum Jakubz., high-molecular-weight glutenin subunits (HMW-GS), SDS-PAGE

中图分类号: 

  • S512.1