关键词: 猪, ESR基因, PCR-RFLP, PvuⅡ多态性

Abstract: Investigation is done on the Polymorphisms of ESR gene in Chinese local swine which include Chenghua pigs, Neijiang pigs and Taihu pigs by use of PCR-RFLPs. The relationship between ESR gene and litter size was analyzed. The results show that the frequencies of allele B in Chenghua pigs, Neijiang pigs and Taihu pigs are 0.6389, 0.6613 and 0.6855 respectively. There are AA, AB and BB genotypes in Neijiang pigs and Taihu pigs, while only AB and BB genotypes are checked in Chenghua pigs. Compared with the final results of the litter size in these three breeds, the litter size of the different genotypes owns certain difference, regardless the first parity or the latter parities, the litter size of three PvuⅡ Polymorphisms of ESR gene shows the tendency of BB > AB > AA in Chenghua pigs, Neijiang pigs and Taihu pigs. That indicates the B allele of ESR is advantageous to the litter size in Chinese local swine. Especially in first parity of Taihu pigs, NBA(number born alive) has significant difference between AB genotype(9.42) and BB genotype(11.13, P<0.05). Prospect and problem of PvuⅡ-RFLP of ESR gene are also analyzed.

Key words: swine, ESR gene, PCR-RFLP, PvuⅡ polymorphism