四川农业大学学报 ›› 2002, Vol. 20 ›› Issue (04): 309-312,320.doi: 10.16036/j.issn.1000-2650.2002.04.002

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应用PCR技术研究四川小麦品种特异性位点遗传多样性

张志清, 郑有良, 魏育明, 吴卫, 周永红, 刘登才, 兰秀锦, 颜泽洪   

  1. 四川农业大学 小麦研究所, 四川 都江堰 611830
  • 收稿日期:2002-04-24 出版日期:2002-12-31 发布日期:2017-03-06
  • 基金资助:
    国家教育部重点项目(00225);四川省教育厅预研项目(2000-C02);四川省科技厅农业生物技术专项基金资助

Genetic Diversity among Sichuan Wheat Cultivars Based on STS-PCR Markers

ZHANG Zhi-qing, ZHENG You-liang, WEI Yu-ming, WU Wei, ZHOU Yong-hong, LIU Deng-cai, LAN Xiu-jin, YAN Ze-hong   

  1. Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, Sichuan, China
  • Received:2002-04-24 Online:2002-12-31 Published:2017-03-06

摘要: 采用PCR技术对四川省近50年来年推广面积达66700hm2(100万亩)以上的40个小麦品种特异性位点(即STS-PCR标记)的遗传多样性进行了研究。13对STS-PCR引物和26种引物-酶组合中,92.3%的引物和88.5%引物-酶组合能揭示小麦品种间的多态性。在检测到的92条DNA片段中,86.96%具有多态性,平均每种引物-酶组合检测到3.08条(变幅为1~8条)。这些品种间遗传相似系数(GS)变幅为0.478~0.989,平均GS值为0.753。STS-PCR标记遗传距离聚类分析能将40个品种相互区分开,其结果与亲缘关系较一致。各年代品种间GS值变化趋势表明,从60年代后四川小麦品种的遗传多样性呈明显的下降趋势。

关键词: 四川小麦, STS-PCR标记, 遗传相似, 聚类分析

Abstract: The genetic diversity among 40 Sichuan wheat(Tritcum aestivum L) cultivars, which has been popularized above 6.67×104 hm2 per year in Sichuan Province during recent 50 years, was investigated by using 26 primer-enzyme combinations of 13 sequence-tagged-site(STS-PCR) primer sets. 12 of 13 primer sets(92.3%) and 23 of 26 primer-enzyme combinations(88.5%) reveal polymorphisms. A total of 92 restriction fragments has been detected in these cultivars, with 3.08 fragments per primer-enzyme combination. The genetic similarity varies from 0.478 to 0.989 among 40 genotypes, with the mean of 0.753. The results show that 40 genotypes can be distinguished by STS-PCR markers. The variation of GS in 5 eras reveals that the genetic similarity has the tendency to increase since 1960.

Key words: SICHUAN WHEAT, STS-PCR, GENETIC SIMILARITY, CHUSTER ANALYSIS

中图分类号: 

  • S512.2