四川农业大学学报 ›› 2001, Vol. 19 ›› Issue (01): 66-69.doi: 10.16036/j.issn.1000-2650.2001.01.017

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里氏木霉利用杂细胞产纤维素酶条件的研究

周江敏, 陈华林, 梁如玉, 漆玉邦   

  1. 四川农业大学 农学院, 四川 雅安 625014
  • 收稿日期:2000-08-31 出版日期:2001-03-15 发布日期:2017-03-06

Study on the Factors of Producing Cellulase by Trichoderma Reesei Using the Parenchyma Cell

ZHOU Jiang-min, CHEN Hua-lin, LIANG Ru-yu, QI Yu-bang   

  1. College of Agriculture, Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2000-08-31 Online:2001-03-15 Published:2017-03-06

摘要: 以里氏木霉(Trichoderma reesei)为产酶菌株,杂细胞为产酶诱导物,通过固态发酵生产纤维素酶。研究结果表明:杂细胞、稻草粉、麸皮三者之比为2:4:4,氮源为硫酸铵(总氮量为0.4%),料水比为1:2,28~30℃恒温培养5d,为最佳产酶条件,CMC酶活达516.64IU/g干曲。在培养基中添加0.1%的表面活性剂Tween 80对产酶无显著影响。纤维素酶作用的最适pH4.85、温度50℃。

关键词: 杂细胞, 里氏木霉, 纤维素酶

Abstract: Cellulase production was carried out by solid state fermentation with Trichoderma reesei when the Parenchyma cell was used for inducers. The results show that the optimum conditions of producing cellulase are:the proportion of parenchyma cell to straw to wheat bran is 2:4:4, the nitrogen is(NH4)2SO4, the water amount of medium is 200%, the temperature is 30℃ and the time is 5 days, in the solid state medium of parenchyma cell, straw powder and wheat bran. The CMCase is as high as 516.64IU/g dry koji. Tween 80 gives a little contribution to CMC ase. The optimal conditions for enzyme action are:pH4.85, 50℃.

Key words: PARENCHYMA CELL, TRICHODERMA REESEI, CELLULASE

中图分类号: 

  • X793