四川农业大学学报 ›› 2000, Vol. 18 ›› Issue (01): 1-3,10.doi: 10.16036/j.issn.1000-2650.2000.01.001

• 研究快报 •    下一篇

新型伪狂犬病病毒基因缺失株的构建及生物学特性研究(初报)

郭万柱, 徐志文, 王小玉, 程陆, 王印, 汪铭书   

  1. 四川农业大学 动物生物技术中心, 四川 雅安 625014
  • 收稿日期:2000-01-21 出版日期:2000-03-31 发布日期:2017-04-21
  • 基金资助:
    国家自然科学基金(批准号:39570544);国家"九五"攻关项目资助。

Construction of new generation Pseudorabies virus gene deleted vaccine and the study of biologic characteristics

GUO Wan-zhu, XU Zhi-wen, Wang Xiao-yu, CHENG Lu, WANG Yin, WANG Ming-shu   

  1. Sichuan Agricultural University, Yaan 625014, Sichuan, China
  • Received:2000-01-21 Online:2000-03-31 Published:2017-04-21

摘要: 以伪狂犬病病毒Fa株为起始材料,构建的pp63LacZ转移载体质粒与PRV Fa DNA经磷酸钙转染,在MDBK细胞内同源重组获得PRV Fa gE-/gI-/LacZ基因缺失株。以PDTK3-6,5-6TK缺失质粒与上述基因缺失株在TK-143细胞内同源重组,获得PRV-SA215等三基因缺失株。经核酸杂交证实构建是成功的。生物学特性观察显示,该基因缺失株对多种动物安全,具有良好的免疫原性,有望成为一株优秀的PRV疫苗候选株。

关键词: 伪狂犬病病毒, 基因缺失, 疫苗, 生物学特性

Abstract: By cotransfection of PRV Fa DNA and the shuttle vector plasmid pp63 LacZ DNA in MDBK cells, a deletion mutant strain PRV Fa gE-/gI-/LacZ was constructed with calcium phosphate transfection system. By cotransfection of PRV Fa gE-/gI-/LacZ DNA and TK gene deleted plasmid PDTK3-6, 5-6 DNA, recombinant viruses were then selected for 5'-BUDR resistance in TK-143 cells and Assayed by using the methods of Dot-Blot hybridization, the results suggest PRV Fa TK-/gE-/gI-/LacZ is constructed successfully, the study of biologic characteristics show that vaccination various animals with the gene deleted strain is safe and effective.

Key words: PSEUDORABIES VIRUS, GENE DELETED, VACCINE, BIOLOGIC CHARACTERISTICS

中图分类号: 

  • S852.655