四川农业大学学报 ›› 1999, Vol. 17 ›› Issue (03): 300-303.doi: 10.16036/j.issn.1000-2650.1999.03.013

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应用Dot-PPA-ELISA检测猪布氏杆菌病抗体的研究

曹三杰, 文心田, 李继祥   

  1. 四川农业大学 动物科技学院, 四川 雅安 625014
  • 收稿日期:1999-07-31 出版日期:1999-09-30 发布日期:2017-04-22

Studies on the Detection of Brucella Antibody of Pig Using Dot-PPA-ELISA

CAO Sanjie, WEN Xintian, LI Jixiang   

  1. SiChuan Agriculture Univesity, Yaan 625014, China
  • Received:1999-07-31 Online:1999-09-30 Published:2017-04-22

摘要: 本试验以布氏杆菌SAT为对照试验,建立了Dot-PPA-ELISA检测猪布氏杆菌病抗体的方法,该法检测的猪IgG含量可达6.992×10-9g,灵敏性高,不同猪抗PRV、PPV、HCV、JEV、CHL等阳性血清发生交叉反应,特异性强;此外该法还具有操作简便、快捷不需特殊试验条件、结果客观、肉眼易于判断、反应膜片可长期保存等优点。适宜在广大基层单位应用,对布病的快速诊检有着十分重要的意义。

关键词: Dot-PPA-ELISA, 猪布氏杆菌病, 抗体

Abstract: A Dot-PPA-ELISA is developed for the pig serum antibodies detection of Brucellosis and SAT of Brucellosis controlled in this studies. The results indicate that Dot-PPA-ELISA is a very sensitive and specific test, the minimum amount of pig sera IgG detected by using Dot-PPA-ELISA ammounts to 6 992×10-9g and its specificity is perfect in crossreaction tests with the positive pig serum of PRV, PPV, HCV, JEV, CHL et al. Many advantages of Dot-PPA-ELISA to detect the Brucella antibodies are described:the test is easy and rapid, no special instrument needed, its results are easy to be determined and the reaction membrain can be stored for a long time et al. In a word, Dot-PPA-ELISA can be uesed widely to detect the Brucella antibodies of pig and has great significance to detect Brucella antibodies quickly.

Key words: Dot-PPA-ELISA, THE PIG BRUCELLOSIS, ANTIBODY

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  • S854.4+3