关键词: 植物激素[45ND, 49LD], 活性炭[49K, 62D, 91B], 聚乙烯吡咯烷酮, C₁₈胶柱, 高压液相色谱, 色素[45ND]

Abstract: Use actived carbon, PVP and Sep-Pak C₁₈ Cartridge as pre-treaments in isolation and purification of phytohormones, dedermine the quantities of phytomones by HPLC. The research results as follow:The metod that treat with Sep-Pak C₁₈ Cartridge is the best one in the three kinds of treatment. After the isolated liquids are treated with Sep-Pak C₁₈ Cartridge, four kinds of phytohorm-ones(IAA, GA, CTK and ABA) can be determined accurately, and its phytohormones quantities have hight rate of retrieving. Although actived carbon can eliminate efficiently chromeons, but it can absorb a lot of phytohormones. The effect of treating with PVP is between acticed carbon and Sep-Pak C₁₈ Cartridge.

Key words: PHYTOHORMONE, ACTIVED CARBON, PVP, SEP-PAK C₁₈ CARTRIDGE, HPLC, PIGMENTS