四川农业大学学报 ›› 1996, Vol. 14 ›› Issue (01): 118-123.doi: 10.16036/j.issn.1000-2650.1996.01.022

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鸭瘟鸭病毒性肝炎二联弱毒疫苗的研究Ⅱ、稳症性、保存条件及保存期的测定

程安春, 汪铭书, 崔恒敏, 廖德惠, 陈孝跃   

  1. 四川农业大学动物科技学院, 雅安市 625014
  • 收稿日期:1995-09-25 出版日期:1996-03-31 发布日期:2017-03-07
  • 基金资助:
    四川省教委资助的重点科研课题

STUDY ON BIVALENT ATTENUATED VACCINES AGAINST DP AND DVH Ⅱ、THE DETECTION OF THE STABILITY, THE CONDITION AND THE PERIOD OF THE BIVALENT ATTENUATED VACCINES PRESERVED

Cheng Anchun, et al   

  1. College of Animal Science and Technology, SichuanAgricultural University/Yaan, Sichuan, China 625014
  • Received:1995-09-25 Online:1996-03-31 Published:2017-03-07

摘要: 本文报道了鸭瘟鸭病毒性肝炎二联弱毒疫苗的稳定性、保存条件和保存期。稳定性的测定是采用了不同保存条件、不同保存期(月)的二联苗,用抗鸭瘟高免血清或抗鸭病毒性肝炎高免血清中和掉二联苗中的相应弱毒后,测定剩下的鸭病毒性肝炎弱毒和鸭瘟弱毒(满度)分别对9日龄鸡压的半数致死置(LD60)和对鸡胚成纤维细胞(CEF)的半数感染量(TCID60)来判定二联苗的稳定性。保存期的测定是采用不同保存条件(℃)不同保存期(月)的二联苗免疫成年鸭,10天后采血测定抗鸭肝炎病毒中和抗体效价及抵抗1万倍最小致死量鸭瘟强毒攻击的能力。试验结果表明:非冻干二联苗子-15——-25℃条件下可保存18个月,0℃冻冻结态下可保存1年,4-10℃可保存半年,15-25℃保存10天。疫苗冻干后,-15——-25℃条件下可保存20个月,0℃保存问个月,4-10℃保存12个月,15-25℃保存20天。

关键词: 鸭瘟鸭病毒性肝炎二联弱毒苗, 稳定性, 保存条件, 保存期, 鸭病[49NA]

Abstract: This paper discusses the stability, the condition and period of bivalent attenuated vaccines against Duck Plague(DP) and Duck Viral Hepatitis(DVH) which should be preserved. The method which was used to detect the stability is to use higt-immune serum ageinst DPV or DHV to neutralize the attenuated DPV or attenuated DHV in the vaccines, then to detect the rest attenuated DHV titers with 9-day chicken embryo(LD60) and the rest attenuated DPV titers with chicken embryo adult-fibre cells(CEF, TCID60) separately. The method used to detect the period of the vaccines which should be preserved is to use the vaccines preserved in different temperature(C) and periods(monthes) to immune the ducks, then to detect the neutralize antibody titers against DHV in the duck serum and the ability with which the immunized ducks can be against the challenge by 10 thousand times of M. L. D. virulent DPV after 10 days. The results indicated that the non-freeze-dried vaccines in -15——-25℃、0℃、4-10℃ and 15-25℃ could be preserved separately for 18 months, 12 months, 6 months and 10 days, and that the freeze-dried bivalent attenuated vaccines in -15——-25℃、0℃、4-10℃ and 15-25℃could be preserved separately for 20 months, 18 months, 12 months and 20 days.

Key words: THE BIVALENT ATTENUATED VACCINES, STABILATY, CONDITICTION, PRESERVED PERIOD PRESERVED, DUCK DISEASE

中图分类号: 

  • S834