关键词: 外源DNA, 变异, 同工酶, 减压渗透, 植物分子育种, 分子遗传

Abstract: Rice seeds were dehulled and soaked in distilled water of 25℃ untill they started germination, The soaking period of about 50 hours could be devided into two phasses:the first was the physical phase of water absorption (about 8h) and the second was the physiological and biochemical phase of embryo germination (about 42h).Water absorbed in these two phases were 65% and 35% of the total respectively.After soaking in water for 4, 14, and 36 hours, the seeds were transferred into DAN solution (500ug/ml) of wheat for 4h with decompress (-0.06Mpa).Then rice seeds were germination under the same tempetrature, Isoenzymes of the rice buds were compared with those of wheat.DNA decrease in the solution was also de determined and cytological observations of roots were made in seedilings of rice treated an d wheat.The results showed that the beat period for exogenous DNA introduction was the second phase of water absorption of the seeds.

Key words: EXOGENOUS DNA OBSORPTION, VARIATION, ISOZYME, DECOMPRESS OSMOSIS, PLANT MOLECULAR BREEDING, MOLECULAR GENETICS