四川农业大学学报 ›› 1994, Vol. 12 ›› Issue (03): 388-391.doi: 10.16036/j.issn.1000-2650.1994.03.010

• • 上一篇    下一篇

减压渗透法将外源DNA导入水稻最佳时期的探讨

刘熔山, 王西瑶, 牛应泽   

  1. 四川农业大学农业科技家院 雅安市 625014
  • 收稿日期:1994-01-24 出版日期:1994-09-30 发布日期:2017-04-22

EXPLORATION OF THE BEST PERIOD FOR INTRODUCTION OF EXOGENOUS DNA TNTO RICE BY DECOMPRESS OSMOSIS

Liu Yongshan, et al   

  1. College of Agricultural Science and Technology, Sichuan Agrianitural Univensity, Yaan, Sichuan, China 625014
  • Received:1994-01-24 Online:1994-09-30 Published:2017-04-22

摘要: 去壳的水稻种子,当浸渍在25℃蒸馏水中至4、14、36个小时,分别转移到小麦DNA溶液内(500mg/ml),施以负压(-0.06Mpa)继续浸渍4小时。在同样温度下催芽,做根尖细胞学观察和幼芽同工酶分析。并测定DNA浸种后的减少量。结果表明:浸渍4、14、36小时后外源DNA减压转导处理的水稻种子,对外源DNA的吸收量依次降低;细胞染色体数目和形状无明显变化;居中者同工酶差异明显,苗期即出现性状变异。减压渗透法将外源DNA导入水稻种子的最佳时期为种子吸水第二阶段的前、中期。

关键词: 外源DNA, 变异, 同工酶, 减压渗透, 植物分子育种, 分子遗传

Abstract: Rice seeds were dehulled and soaked in distilled water of 25℃ untill they started germination, The soaking period of about 50 hours could be devided into two phasses:the first was the physical phase of water absorption (about 8h) and the second was the physiological and biochemical phase of embryo germination (about 42h).Water absorbed in these two phases were 65% and 35% of the total respectively.After soaking in water for 4, 14, and 36 hours, the seeds were transferred into DAN solution (500ug/ml) of wheat for 4h with decompress (-0.06Mpa).Then rice seeds were germination under the same tempetrature, Isoenzymes of the rice buds were compared with those of wheat.DNA decrease in the solution was also de determined and cytological observations of roots were made in seedilings of rice treated an d wheat.The results showed that the beat period for exogenous DNA introduction was the second phase of water absorption of the seeds.

Key words: EXOGENOUS DNA OBSORPTION, VARIATION, ISOZYME, DECOMPRESS OSMOSIS, PLANT MOLECULAR BREEDING, MOLECULAR GENETICS

中图分类号: 

  • S51141