四川农业大学学报 ›› 1994, Vol. 12 ›› Issue (02): 289-292.doi: 10.16036/j.issn.1000-2650.1994.02.024

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狂犬病(HEP/BHK21活疫苗的试制

郭万柱, 冯炳芳, 蒋文灿, 赵景芬, 庄冬跃, 谢跃, 余广海, 汪铭书   

  1. 四川农业大学/动物科技学院, 雅安市 625014
  • 收稿日期:1993-05-30 出版日期:1994-06-30 发布日期:2017-04-22

STUDIES ON THE PRODUCTION OF RABIES LIVE VACCINE (HEP/BHK21)

Guo Wanzhu, et al   

  1. College of Animal Science and Technology Sichuan Agricultural University, Yaan, Sichuan, China 625014
  • Received:1993-05-30 Online:1994-06-30 Published:2017-04-22

摘要: 狂犬病FluryHEP株经BHK21细胞传代后,从1987年试制了7批疫苗,批间毒价稳定,MICLD50均在10-4.50/0.03ml以上,冻干苗毒价≥10-4.50/0.03ml。冻干苗以5%蔗糖、1%明胶为保护剂,在-20℃保存一年,4-8℃保存一年,室温(18~25℃)保存7天,毒价均无明显下降。

关键词: 狂犬病(47MA), 冻干活疫菌, 抗体(47KD), 检测(52HB58BC)

Abstract: Seven lot of modified live virus rabies vaccine were prepared by growing HEP strain on BHk21 cell cultures from 1987 The titer of the vaccine was stable at different lot.A lyophilized live vaecine was prepared with 5% sucross and 15 glatine as stabilizer.The titer of this vaccine was usually ≥ 10-4.50 MICLD50/0.03ml, It was not significantly reduced after keeping the vaccine at-20℃ and 4-8℃ for one yeay, or at room temperature for 7 days.

Key words: RABIES, LYOPHILIZED LIVE VACCINE, ANTIBODIES, DETECTION

中图分类号: 

  • S855.3