四川农业大学学报 ›› 1994, Vol. 12 ›› Issue (02): 285-288.doi: 10.16036/j.issn.1000-2650.1994.02.023

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应用ABC-ELISA和SPA-ELISA检测狂犬病疫苗免疫犬后血清中的抗体水平

郭万柱, 曹军, 孔树全, 冯炳芳, 蒋文灿   

  1. 四川农业大学动物科技学院, 雅安市 625014
  • 收稿日期:1993-08-30 出版日期:1994-06-30 发布日期:2017-04-22

DETECTION OF ANTIBODIES FROM DOGS VACCINATED WITH RABIES VACCINE BY ABC-ELISA AND SPA-ELISA

Guo Wanzhu, et al   

  1. College of Animal Science and Technology Sichuan Agricultural University, Yaan, Sichuan, China 625014
  • Received:1993-08-30 Online:1994-06-30 Published:2017-04-22

摘要: 本文报道应用ABC-ELISA和SPA-ELISA检测狂犬病疫苗免疫犬后血清中抗体的效价,比较研究了两种方法的特异性,敏感性和重复性,结果显示两种方法均适用于检测狂犬病血清抗体水平。ABC-ELISA较SPA-ELISA敏感性高4倍,前者的阳性检出率(100%)高于后者(92.8%)。

关键词: 狂犬病(47MA), 抗体(47KD), 检测(52HB58BC)

Abstract: This paper reported detection of antibodies from dogs vaccinated with rabies vaccine by ABC-ELISA and SPA-ELISA, Thespecifiaty, sensitivity and stability of both mothods were compared.The results showed that both methods were applicable todetccting the level of antibody to rabies virus.The sensitivity of ABC-ELISA was four times as much as that of SPA-ELSA.Thepositive rate (100%) in ABC-ELISA was higher than that (92.8%) in SPA-ELISA.

Key words: RABIES, ANTIBODIES, DETECTION

中图分类号: 

  • S855.3